His Holiness received his degree in pharmacy in 1975 from the University of Alexandria and in 1985, earned a fellowship of the World Health Organization from the British International Health Institute in England. In his last year at the University of Alexandria, he enrolled at the Coptic Orthodox Theological Seminary of Alexandria, where he graduated in 1983. His professional career in pharmacy led him back to Damanhour where he managed a pharmaceutical company of the Egyptian Ministry of Health. Pope Tawadros' close work with Metropolitan Pachomius, Archbishop of Beheira, goes back to his years as a university student when his broadmindedness and keenness on church service drew the archbishop's attention. He served with the Metropolitan until he took to monastic life, and in 1986 went to the Monastery of St. Bishoy in Wadi El-Natrun.
Following the departure of H.H. Pope Shenouda III on March 17, 2012, H.E. Metropolitan Pachomius was unanimously elected Locum Tenens (Acting Patriarch) of the Throne of St. Mark. According to the practices and traditions of the Coptic Orthodox Church, following the participation of clergy and laity in elections, three names of the final candidates for the papacy were placed in the Altar Lot on Sunday, November 4, 2012.
The name of H.G. Bishop Tawadros was selected and His Holiness was enthroned as Pope Tawadros II on November 18th, 2012 at the Grand Cathedral of St. Mark in Cairo, Egypt.
On May 10, 2013, Pope Tawadros II paid a fraternal visit to Pope Francis of Rome at the Vatican. This visit, his first international visit since his enthronement, was timed to commemorate the 40th anniversary of the visit of Pope Shenouda III to Pope Paul VI in 1973.
In September 2014, His Holiness visited Ottawa, Canada. He led the liturgy at St. George and St. Anthony Coptic Orthodox Church, in which he added new deacons, baptized three children, and blessed the church's third priest, Abouna Anthony.